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Mammalian cells consist of a cellular membrane surrounding groups of specialized organelles. It is these sub-cellular organelles that differentiate eukaryotes from prokaryotes like bacteria. The organelles also provide a variety of targets for poisons or drugs. In many cases, the same chemical is poisonous to healthy cells at high doses or produces therapeutic effects at lower doses. Cytotoxic chemotherapeutic agents lack even this distinction and rely on malignant cells that replicate rapidly and accumulate the agent and die before healthy cells suffer excess toxicity. Methotrexate is an antimetabolite cytotoxic drug that binds to the enzyme that activates folic acid in the synthesis of purine bases for DNA. In high doses, healthy human cells need folinic acid (an alternative pathway for purines) to rescue them from excess toxicity.
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